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Esposito, F., Sinquin, C., Colliec-Jouault, S., Cuenot, S., Pugnière, M., Ngo, G., Traboni, S., Zykwinska, A. & Bedini, E. (2024) Multi-step semi-synthesis, structural characterization and growth factor interaction study of regiochemically sulfated diabolican polysaccharides. International Journal of Biological Macromolecules, 260 129483.
Added by: Richard Baschera (2024-04-16 07:57:03) Last edited by: Richard Baschera (2024-04-16 08:05:03) |
| Type de référence: Article DOI: 10.1016/j.ijbiomac.2024.129483 Numéro d'identification (ISBN etc.): 0141-8130 Clé BibTeX: Esposito2024 Voir tous les détails bibliographiques  |
Catégories: INTERNATIONAL, PMN Mots-clés: Growth factors, Marine polysaccharides, Multi-step semi-synthesis, regioselectivity, Sulfation Créateurs: Bedini, Colliec-Jouault, Cuenot, Esposito, Ngo, Pugnière, Sinquin, Traboni, Zykwinska Collection: International Journal of Biological Macromolecules |
Consultations : 3/3
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| Liens URLs https://www.scienc ... /S0141813024002861 |
| Résumé |
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Diabolican is an exopolysaccharide (EPS) produced by Vibrio diabolicus HE800, a mesophilic bacterium firstly isolated from a deep-sea hydrothermal field. Its glycosaminoglycan (GAG)-like structure, consisting of a tetrasaccharide repeating unit composed of two aminosugars (N-acetyl-glucosamine and N-acetyl-galactosamine) and two glucuronic acid units, suggested to subject it to regioselective sulfation processes, in order to obtain some sulfated derivatives potentially acting as GAG mimics. To this aim, a multi-step semi-synthetic approach, relying upon tailored sequence of regioselective protection, sulfation and deprotection steps, was employed in this work. The chemical structure of the obtained sulfated diabolican derivatives was characterized by a multi-technique analytic approach, in order to define both degree of sulfation (DS) and sulfation pattern within the polysaccharide repeating unit, above all. Finally, binding affinity for some growth factors relevant for biomedical applications was measured for both starting diabolican and sulfated derivatives thereof. Collected data suggested that sulfation pattern could be a key structural element for the selective interaction with signaling proteins not only in the case of native GAGs, as already known, but also for GAG-like structures obtained by regioselective sulfation of naturally unsulfated polysaccharides.
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