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Fillaudeau, A., Cuenot, S., Makshakova, O., Traboni, S., Sinquin, C., Hennetier, M., Bedini, E., Perez, S., Colliec-Jouault, S. & Zykwinska, A. (2024) Glycosaminoglycan-mimetic infernan grafted with poly(N-isopropylacrylamide): Toward a thermosensitive polysaccharide. Carbohydrate Polymers, 326 121638.
Added by: Richard Baschera (2024-01-19 15:14:15) Last edited by: Richard Baschera (2024-01-19 15:26:42) |
| Type de référence: Article DOI: 10.1016/j.carbpol.2023.121638 Numéro d'identification (ISBN etc.): 0144-8617 Clé BibTeX: Fillaudeau2024 Voir tous les détails bibliographiques  |
Catégories: INTERNATIONAL, PMN Mots-clés: afm, exopolysaccharide, Molecular dynamics, Monte Carlo Simulations, NMR, pNIPAM Créateurs: Bedini, Colliec-Jouault, Cuenot, Fillaudeau, Hennetier, Makshakova, Perez, Sinquin, Traboni, Zykwinska Collection: Carbohydrate Polymers |
Consultations : 1/52
Indice de consultation : 10% Indice de popularité : 2.5% |
| Liens URLs https://www.scienc ... /S0144861723011037 |
| Résumé |
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Glycosaminoglycans (GAGs) are essential constituents of the cell surface and extracellular matrix, where they are involved in several cellular processes through their interactions with various proteins. For successful tissue regeneration, developing an appropriate matrix supporting biological activities of cells in a similar manner than GAGs remains still challenging. In this context, this study aims to design a thermosensitive polysaccharide that could further be used as hydrogel for tissue engineering applications. For this purpose, infernan, a marine bacterial exopolysaccharide (EPS) endowed with GAG-mimetic properties was grafted with a thermosensitive polymer, poly(N-isopropylacrylamide) (pNIPAM). Eight grafted polysaccharides were obtained by varying EPS/pNIPAM molar ratio and the molecular weight of pNIPAM. Their physicochemical characteristics and their thermosensitive properties were determined using a multi-technique, experimental approach. In parallel, molecular dynamics and Monte Carlo simulations were applied at two different scales to elucidate, respectively, the molecular conformation of grafted infernan chain and their ability to form an infinite network undergoing a sol-gel transition near the percolation, a necessary condition in hydrogel formation. It comes out from this study that thermosensitive infernan was successfully developed and its potential use in tissue regeneration as a hydrogel scaffold will further be assessed.
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