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Norris, K., Mishukova, O. I., Zykwinska, A., Colliec-Jouault, S., Sinquin, C., Koptioug, A., Cuenot, S., Kerns, J. G., Surmeneva, M. A., Surmenev, R. A. & Douglas, T. E. L. (2019) Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. Micromachines, 10 68.
Added by: Richard Baschera (2019-03-26 10:55:33) Last edited by: Richard Baschera (2019-03-26 11:05:38) |
| Type de référence: Article DOI: 10.3390/mi10010068 Clé BibTeX: Norris2019 Voir tous les détails bibliographiques  |
Catégories: INTERNATIONAL, PMN Créateurs: Colliec-Jouault, Cuenot, Douglas, Kerns, Koptioug, Mishukova, Norris, Sinquin, Surmenev, Surmeneva, Zykwinska Collection: Micromachines |
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| Résumé |
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Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.
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