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Le Guehennec, L., Martin, F., Lopez-Heredia, M.-A., Louarn, G., Amouriq, Y., Cousty, J. & Layrolle, P. (2008) Osteoblastic cell behavior on nanostructured metal implants. Nanomedicine, 3 61–71.
Added by: Laurent Cournède (2016-03-10 21:58:42) |
| Type de référence: Article DOI: 10.2217/17435889.3.1.61 Numéro d'identification (ISBN etc.): 1743-5889 Clé BibTeX: LeGuehennec2008 Voir tous les détails bibliographiques  |
Catégories: PMN Mots-clés: Adhesion, biomaterials, cell adhesion, cell differentiation, differentiation, expression, in-vitro, kinase, nanosrructuration, Osteoblasts, proliferation, stainless steel, surface-energy, ti-6al-4v, topography Créateurs: Amouriq, Cousty, Layrolle, Le Guehennec, Lopez-Heredia, Louarn, Martin Collection: Nanomedicine |
Consultations : 1/534
Indice de consultation : 4% Indice de popularité : 1% |
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Aims: Surface modifications at the nanometric scale may promote protein adsorption, cell adhesion and thus favor the osseointegration of metal implants. The behavior of osteoblastic cells was studied on mirror-polished (Smooth-SS) and nanostructured (Nano-SS) stainless steel surfaces. Materials \& methods: The nanostructuration was obtained by anodization. The surfaces were characterized by scanning electron, atomic force microscopy (AFM) and X-ray photoelectron specytoscopy. Osteoblastic cells were cultured on these surfaces. Results \& discussion: AFM showed a regular array of pores on the Nano-SS. Osteoblastic cells spread more rapidly on Nano-SS surfaces than on Smooth-SS surfaces. Cell viability was similar on Smooth-SS and Nano-SS. Alkaline phosphatase activity increased with culture time whatever the substrate but was better enhanced at 21 days on the Nano-SS than on the Smooth-SS. Conclusion: This study shows that the nanostructuration of metal implants may improve the adhesion and differentiation of osteoblastic cells.
Added by: Laurent Cournède |