Tesson, B., Genet, M. J., Fernandez, V., Degand, S., Rouxhet, P. G. & Martin-Jezequel, V. (2009) Surface Chemical Composition of Diatoms. ChemBioChem, 10 2011–2024.
Added by: Laurent Cournède (2016-03-10 21:41:23)
|Type de référence: Article
Numéro d'identification (ISBN etc.): 1439-4227
Clé BibTeX: Tesson2009
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Mots-clés: azospirillum-brasilense, Biomineralization, cell-walls, diatoms, fine structure, phaeodactylum-tricornutum bacillariophyceae, silica, silica shell formation, silicates, spectroscopy analysis, thalassiosira-pseudonana, x-ray photoelectron, X-ray photoelectron spectroscopy, xps analysis, yeast-cells
Créateurs: Degand, Fernandez, Genet, Martin-Jezequel, Rouxhet, Tesson
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Among diatoms, Phaeodactylum tricornutum is a peculiar species that exists in three morphotypes with distinct cell wall structures and low silica context. X-ray photoelectron spectroscopy (XPS) analysis was performed on P. tricornutum and compared with diatom Thalassiosira pseudonana, the result provide new information on the chemical composition (elements, chemical functions, classes of biochemical compounds) of the cell surface. Two types of silicon were found condensed-silica (SiO(2)) and weakly polymerised silicate. Cells of T. pseudonana showed the highest concentration of silicon, with a majority in the form of condensed silica. For the fusiform and triradiate morpotypes of P. tricornutum, the majority of the small concentration of silican found was in the form of weakly polymerised silica. for all morphotypes of P. tricornutum, higher polysaccharide concentrations replaced silicas as a structural part of the cell wall. In both diatoms a high concentration of lipids was measured, in the form of carboxylic esters. Protonated nitrogen and phosphate were found in correlated amounts and attributed not only to phospholipids but also to phosphoproteins. Chloride ions characterised by a high electron density might be associated to the moieties. Sulfate groups were also detected, principally in P. tricornutum, and attributed to monoesters of polysaccharides.
Added by: Laurent Cournède