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Roussille, L., Brotons, G., Ballut, L., Louarn, G., Ausserre, D. & Ricard-Blum, S. (2011) Surface characterization and efficiency of a matrix-free and flat carboxylated gold sensor chip for surface plasmon resonance (SPR). Anal. Bioanal. Chem. 401 1601–1617.
Added by: Laurent Cournède (2016-03-10 21:32:20) |
| Type de référence: Article DOI: 10.1007/s00216-011-5220-z Numéro d'identification (ISBN etc.): 1618-2642 Clé BibTeX: Roussille2011 Voir tous les détails bibliographiques  |
Catégories: PMN Mots-clés: au(111), biosensor, biosensors, carbodiimide, endostatin, gold surface, heparan-sulfate, immobilization, kinetics, protein adsorption, protein-protein interaction, Self-assembled monolayer, self-assembled monolayers, spectroscopy, Surface characterization, surface plasmon resonance Créateurs: Ausserre, Ballut, Brotons, Louarn, Ricard-Blum, Roussille Collection: Anal. Bioanal. Chem. |
Consultations : 1/555
Indice de consultation : 4% Indice de popularité : 1% |
| Résumé |
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We report the preparation and characterization of a matrix-free carboxylated surface plasmon resonance (SPR) sensor chip with high sensing efficiency by functionalizing a bare gold thin film with a self-assembled monolayer of 16-mercaptohexadecanoic acid (SAM-MHDA chip). The self assembled monolayer surface coverage of the gold layer was carefully evaluated and the SAM was characterized by infrared reflection absorption spectroscopy, X-ray photoemission spectroscopy, atomic force microscopy, X-ray reflectivity-diffraction, and SPR experiments with bovine serum albumin. We compared the SPR signal obtained on this chip made of a dense monolayer of carboxylic acid groups with commercially available carboxylated sensor chips built on the same gold substrate, a matrix-free C1 chip, and a CM5 chip with a similar to 100 nm dextran hydrogel matrix (GE Healthcare). Two well-studied interaction types were tested, the binding of a biotinylated antibody (immunoglobulin G) to streptavidin and an antigen-antibody interaction. For both interactions, the well characterized densely functionalized SAM-MHDA chip gave a high signal-to-noise ratio and showed a gain in the availability of immobilized ligands for their partners injected in buffer flow. It thus compared favourably with commercially available sensor chips.
Added by: Laurent Cournède |